On Tuesday April 13, 2004, 4:00 PM Barus & Holley (Room 190) Dr. Shinya Inoue (Marine Biological Laboratory, Woods Hole) presented a talk entitled:

"Exploring Fine-structural and Molecular Dynamics in Living Cells with Polarized Light Microscopy"

as part of the Seminar Series

"Frontiers in the Interaction Between Physics and Biology"

Abstract:

Drawing on 60 years of experience, I plan to demonstrate: the reasons for using polarized light for studying living cells; what special features are needed in order to make useful observations and measurements with a microscope employing polarized light; and examples of fine-structural and molecular dynamics in dividing cells and early embryogenesis that we have learned, or should be able to learn, by analysis with polarization light microscopy.

References:
Hartshorne, N. H., and A. Stuart (1960) Crystals and the Polarizing Microscope: A Handbook for Chemists and Others, 3rd ed., Arnold, London.
Inoué, S. (1986) Video Microscopy, 1st ed., Plenum Press, New York, Appendix III.
Inoué, S. et al (2001) Centrifuge polarizing microscope. I. Rational, design, and instrument performance. J. Microscopy 201: 341-356. II. Sample biological applications. J. Microscopy 201: 357-367.
Inoué, S. et al (2002) Fluorescence polarization of green fluorescent protein (GFP). Proc. Natl. Acad. Sci. USA 99(7): 4272-4277 and PNAS Online Supporting Information.
Inoué, S. (2003) Polarization microscopy Brown. In J. Lippincott-Schwartz ed. Current Protocols in Cell Biology, Vol. 1, Suppl. 13. John Wiley & Sons, pp.4.9.1-4.9.27.